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anti mouse cd11b pe  (Biogems International)


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    Structured Review

    Biogems International anti mouse cd11b pe
    Anti Mouse Cd11b Pe, supplied by Biogems International, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd11b/Anti-Human%2FMouse+CD11b+PE/pm42892647-195-21-24
    Average 93 stars, based on 7 article reviews
    anti mouse cd11b pe - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: Single-cell RNA sequencing of endometrium uncovers dynamic characteristics and dysregulation of perivascular CD9 + SUSD2 + cells in thin endometrium
    Article Snippet: In total, 1 × 10 5 cells were incubated with 1% bovine serum albumin (BSA) (Cat# A8020, Solarbio, China)/DPBS (Cat# 14190250, Gibco, USA) for 30 min to block nonspecific antigens. .. Subsequently, the cells were incubated in the dark with fluorescein isothiocyanate (FITC)-labeled anti-CD73 (Cat# 10904-MM07-F, Sino Biological, China), CD90 (Cat# 16897-MM10-F, Sino Biological, China), CD105 (Cat# 561443, BD Pharmingen, USA), CD34 (Cat# 68035-XM01-F, Sino Biological, China), CD45 (Cat# 10086-MM05-F, Sino Biological, China), CD11b (Cat# 03211-50, BioGems, USA), CD19 (Cat# 11880-MM17-F, Sino Biological, China), HLA-DR (Cat# 68038-XM01-F, Sino Biological, China), and Isotype Control FITC (Cat# 44212-50, BioGems, USA) at room temperature for 30 min. ..

    Article Title: Single-cell RNA sequencing of endometrium uncovers dynamic characteristics and dysregulation of perivascular CD9 + SUSD2 + cells in thin endometrium.
    Article Snippet: In total, 1 × 105 cells were incubated with 1% bovine serum albumin (BSA) (Cat# A8020, Solarbio, China)/DPBS (Cat# 14190250, Gibco, USA) for 30 min to block nonspecific antigens. .. Subsequently, the cells were incubated in the dark with fluorescein isothiocyanate (FITC)-labeled anti-CD73 (Cat# 10904-MM07-F, Sino Biological, China), CD90 (Cat# 16897-MM10-F, Sino Biological, China), CD105 (Cat# 561443, BD Pharmingen, USA), CD34 (Cat# 68035-XM01-F, Sino Biological, China), CD45 (Cat# 10086-MM05-F, Sino Biological, China), CD11b (Cat# 03211-50, BioGems, USA), CD19 (Cat# 11880-MM17-F, Sino Biological, China), HLA-DR (Cat# 68038-XM01-F, Sino Biological, China), and Isotype Control FITC (Cat# 44212-50, BioGems, USA) at room temperature for 30 min. ..

    Control:

    Article Title: Single-cell RNA sequencing of endometrium uncovers dynamic characteristics and dysregulation of perivascular CD9 + SUSD2 + cells in thin endometrium
    Article Snippet: In total, 1 × 10 5 cells were incubated with 1% bovine serum albumin (BSA) (Cat# A8020, Solarbio, China)/DPBS (Cat# 14190250, Gibco, USA) for 30 min to block nonspecific antigens. .. Subsequently, the cells were incubated in the dark with fluorescein isothiocyanate (FITC)-labeled anti-CD73 (Cat# 10904-MM07-F, Sino Biological, China), CD90 (Cat# 16897-MM10-F, Sino Biological, China), CD105 (Cat# 561443, BD Pharmingen, USA), CD34 (Cat# 68035-XM01-F, Sino Biological, China), CD45 (Cat# 10086-MM05-F, Sino Biological, China), CD11b (Cat# 03211-50, BioGems, USA), CD19 (Cat# 11880-MM17-F, Sino Biological, China), HLA-DR (Cat# 68038-XM01-F, Sino Biological, China), and Isotype Control FITC (Cat# 44212-50, BioGems, USA) at room temperature for 30 min. ..

    Article Title: Single-cell RNA sequencing of endometrium uncovers dynamic characteristics and dysregulation of perivascular CD9 + SUSD2 + cells in thin endometrium.
    Article Snippet: In total, 1 × 105 cells were incubated with 1% bovine serum albumin (BSA) (Cat# A8020, Solarbio, China)/DPBS (Cat# 14190250, Gibco, USA) for 30 min to block nonspecific antigens. .. Subsequently, the cells were incubated in the dark with fluorescein isothiocyanate (FITC)-labeled anti-CD73 (Cat# 10904-MM07-F, Sino Biological, China), CD90 (Cat# 16897-MM10-F, Sino Biological, China), CD105 (Cat# 561443, BD Pharmingen, USA), CD34 (Cat# 68035-XM01-F, Sino Biological, China), CD45 (Cat# 10086-MM05-F, Sino Biological, China), CD11b (Cat# 03211-50, BioGems, USA), CD19 (Cat# 11880-MM17-F, Sino Biological, China), HLA-DR (Cat# 68038-XM01-F, Sino Biological, China), and Isotype Control FITC (Cat# 44212-50, BioGems, USA) at room temperature for 30 min. ..



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    ( A ) Experimental timeline. ( B ) Representative confocal images at D4, D10, D30, and D45 showing that GFP is not co-labeled with TUJ1 or MAP2 in the brain. ( C ) Quantification of TUJ1 + GFP + double-positive cells among TUJ1 + cells at D4, D10, D30, and D45. N = 4 or 5 mice for each group. ( D ) Quantification of MAP2 + GFP + double-positive cells among MAP2 + cells at D4, D10, D30, and D45. N = 4 or 5 mice for each group. ( E ) Relative gene expression level at D18. N = 4 mice for each group. Data are presented as mean ± SD. Each data point represents one mouse.
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    Image Search Results


    ( A ) Experimental timeline. ( B ) Representative confocal images at D4, D10, D30, and D45 showing that GFP is not co-labeled with TUJ1 or MAP2 in the brain. ( C ) Quantification of TUJ1 + GFP + double-positive cells among TUJ1 + cells at D4, D10, D30, and D45. N = 4 or 5 mice for each group. ( D ) Quantification of MAP2 + GFP + double-positive cells among MAP2 + cells at D4, D10, D30, and D45. N = 4 or 5 mice for each group. ( E ) Relative gene expression level at D18. N = 4 mice for each group. Data are presented as mean ± SD. Each data point represents one mouse.

    Journal: eLife

    Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons

    doi: 10.7554/eLife.112083

    Figure Lengend Snippet: ( A ) Experimental timeline. ( B ) Representative confocal images at D4, D10, D30, and D45 showing that GFP is not co-labeled with TUJ1 or MAP2 in the brain. ( C ) Quantification of TUJ1 + GFP + double-positive cells among TUJ1 + cells at D4, D10, D30, and D45. N = 4 or 5 mice for each group. ( D ) Quantification of MAP2 + GFP + double-positive cells among MAP2 + cells at D4, D10, D30, and D45. N = 4 or 5 mice for each group. ( E ) Relative gene expression level at D18. N = 4 mice for each group. Data are presented as mean ± SD. Each data point represents one mouse.

    Article Snippet: The cell pellet was then resuspended in 90 μl of 0.5% BSA in DPBS and incubated with 10 μl of anti-CD11b magnetic beads (Miltenyi, Cat#: 130-097-142) for 30 min at 4°C.

    Techniques: Confocal, Labeling, Gene Expression, Magnetic Cell Separation, Real-time Polymerase Chain Reaction

    Using qPCR verify the relative expression of Neurod1 .

    Journal: eLife

    Article Title: In vivo virus-free lineage tracing and live imaging reveal that NeuroD1 does not reprogram microglia into neurons

    doi: 10.7554/eLife.112083

    Figure Lengend Snippet: Using qPCR verify the relative expression of Neurod1 .

    Article Snippet: The cell pellet was then resuspended in 90 μl of 0.5% BSA in DPBS and incubated with 10 μl of anti-CD11b magnetic beads (Miltenyi, Cat#: 130-097-142) for 30 min at 4°C.

    Techniques: Expressing, Gene Expression

    G31P–BPI 233 limits neutrophil-associated inflammation during CRAB pneumonia. Mice were intranasally infected with A. baumannii ATCC BAA-1605 and treated with saline, G31P, BPI 233 , an equimolar G31P + BPI 233 mixture, G31P–BPI 233 , or colistin (n = 5 per group). Uninfected mice served as normal controls (n = 3). Samples were collected 24 h after infection. (A–D) Representative flow-cytometry plots and quantification of CD45⁺CD11b⁺Ly6G⁺ neutrophils among lung (A, B) and peripheral blood (C, D) leukocytes. (E, F) Pulmonary levels of MPO and extracellular dsDNA. Component doses were molar-matched to the corresponding fusion-protein dose. Data are presented as mean ± SEM. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

    Journal: Emerging Microbes & Infections

    Article Title: A novel CXCL8(3–72) K11R/G31P–BPI 233 hybrid protein modulates neutrophil-driven inflammation and protects against carbapenem-resistant Acinetobacter baumannii infection

    doi: 10.1080/22221751.2026.2741787

    Figure Lengend Snippet: G31P–BPI 233 limits neutrophil-associated inflammation during CRAB pneumonia. Mice were intranasally infected with A. baumannii ATCC BAA-1605 and treated with saline, G31P, BPI 233 , an equimolar G31P + BPI 233 mixture, G31P–BPI 233 , or colistin (n = 5 per group). Uninfected mice served as normal controls (n = 3). Samples were collected 24 h after infection. (A–D) Representative flow-cytometry plots and quantification of CD45⁺CD11b⁺Ly6G⁺ neutrophils among lung (A, B) and peripheral blood (C, D) leukocytes. (E, F) Pulmonary levels of MPO and extracellular dsDNA. Component doses were molar-matched to the corresponding fusion-protein dose. Data are presented as mean ± SEM. ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

    Article Snippet: Human neutrophils were identified as CD45+CD16+CD66b+ cells using the following antibodies: anti-human CD45 (BioLegend, Cat# 368526), anti-human CD16 (BioLegend, Cat# 980102), and anti-human CD66b (BioLegend, Cat# 305108) [ ], and mouse BM neutrophils as CD45+Ly6G+CD11b+ cells using the following antibodies: anti-CD11b (Miltenyi Biotec, Cat# 130-113-805), anti-Ly6G (Miltenyi Biotec, Cat# 130-119-126), and anti-CD45 (Miltenyi Biotec, Cat# 130-110-803) [ ].

    Techniques: Infection, Saline, Flow Cytometry